Measurement of macrophage growth and differentiation
V Chitu, YG Yeung, W Yu, S Nandi… - Current protocols in …, 2011 - Wiley Online Library
V Chitu, YG Yeung, W Yu, S Nandi, ER Stanley
Current protocols in immunology, 2011•Wiley Online LibraryThis unit provides protocols for measuring the abundance and growth of macrophage
precursors in agar cultures and the proliferation of isolated mature macrophages in vitro, by
either direct cell counting or by DNA measurement. Methods for the immunohistochemical
identification of macrophages and the determination of their proliferative status in vivo by
immunofluorescence are also included. It also describes methods for characterization of
macrophage differentiation through the immunofluorescence analysis of cell‐surface …
precursors in agar cultures and the proliferation of isolated mature macrophages in vitro, by
either direct cell counting or by DNA measurement. Methods for the immunohistochemical
identification of macrophages and the determination of their proliferative status in vivo by
immunofluorescence are also included. It also describes methods for characterization of
macrophage differentiation through the immunofluorescence analysis of cell‐surface …
Abstract
This unit provides protocols for measuring the abundance and growth of macrophage precursors in agar cultures and the proliferation of isolated mature macrophages in vitro, by either direct cell counting or by DNA measurement. Methods for the immunohistochemical identification of macrophages and the determination of their proliferative status in vivo by immunofluorescence are also included. It also describes methods for characterization of macrophage differentiation through the immunofluorescence analysis of cell‐surface expression of CSF‐1 receptor. Curr. Protoc. Immunol. 92:14.20.1‐14.20.26. © 2011 by John Wiley & Sons, Inc.
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