[HTML][HTML] Sarcolipin inhibits polymerization of phospholamban to induce superinhibition of sarco (endo) plasmic reticulum Ca2+-ATPases (SERCAs)
M Asahi, K Kurzydlowski, M Tada… - Journal of Biological …, 2002 - ASBMB
Sarcolipin (SLN), a regulator of the sarco (endo) plasmic reticulum Ca 2+-ATPase of fast-
twitch skeletal muscle (SERCA1a), is also expressed in cardiac and slow-twitch skeletal
muscles where phospholamban (PLN) and SERCA2a are expressed. Co-expression in HEK-
293 cells of SLN tagged N-terminally with a FLAG epitope (NF-SLN), PLN, and SERCAs
followed by measurement of the Ca 2+ dependence of Ca 2+ transport activity in isolated
microsomal fractions showed that NF-SLN can reduce the apparent Ca 2+ affinity of both …
twitch skeletal muscle (SERCA1a), is also expressed in cardiac and slow-twitch skeletal
muscles where phospholamban (PLN) and SERCA2a are expressed. Co-expression in HEK-
293 cells of SLN tagged N-terminally with a FLAG epitope (NF-SLN), PLN, and SERCAs
followed by measurement of the Ca 2+ dependence of Ca 2+ transport activity in isolated
microsomal fractions showed that NF-SLN can reduce the apparent Ca 2+ affinity of both …
